Journal article
Colon organoid formation and cryptogenesis are stimulated by growth factors secreted from myofibroblasts
H Yan Kelvin Yip, CW Tan, Y Hirokawa, AW Burgess
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2018
Abstract
Although small intestinal epithelial stem cells form crypts when using intestinal culture conditions, colon stem cells usually form colonospheres. Colon mesenchymal cell feeder layers can stimulate colon crypts to form organoids and produce crypts. We have investigated whether conditioned medium from colon mesenchymal cells can also stimulate colonosphere and organoid cryptogenesis. We prepared conditioned medium (CM) from WEHI-YH2 cells (mouse colon myofibroblasts); the CM stimulated both colonosphere formation and organoid cryptogenesis in vitro. The colon organoid-stimulating factors in WEHI-YH2 CM are inactivated by heating and trypsin digestion and proteins can be concentrated by ultraf..
View full abstractGrants
Awarded by Ludwig Institute for Cancer Research
Funding Acknowledgements
This work was supported by the Ludwig Institute for Cancer Research, the National Health and Medical Research Council (NHMRC) Project Grant GNT1029628 and, Program Grant #487922, and funds from the Operational Infrastructure Support Program provided by the Victorian Government, Australia. The funders had no role on the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The manuscript is solely the responsibility of the institutions and individual authors and does not reflect the views of the NHMRC. H.Y.K.Y was supported by the Melbourne International Research Scholarship and Melbourne International Fee Remission Scholarship.